Journal: PLoS ONE

Article Title: Trends and determinants of gastric bacterial colonization of preterm neonates in a NICU setting

doi: 10.1371/journal.pone.0114664

Figure Lengend Snippet: Number of bacterial species detected in gastric aspirate samples of preterm neonates during the first four weeks of life, by feeding type. The X-axis in each graph represents individual bacterial species, and the Y-axis in each graph represents the total number of bacterial species. Partially breast milk fed infants were fed breast milk and preterm formula. * Bacteroides spp . includes Bacteroides fragilis and Bacteroides thetaiotaomicron; ** Lactobacillus spp . includes Lactobacillus plantarum and Lactobacillus acidophilus . Other includes DGGE bands that did not correspond to the ATCC standards in the marker. Bacterial species were counted based on DGGE bands.

Article Snippet: All ATCC strain DNA amplified 16S rDNA V3-amplicons (~200bp), and resulted in a sequence specific band resolution in DGGE (35% to 55% gradient gel), forming a DNA ladder (Marker lane, ) in line with- Bacteroides thetaiotaomicron (ATCC 29148D-5), Bacteroides fragilis (ATCC 25285D), Lactobacillus plantarum (ATCC BAA-793D-5), Staphylococcus aureus (ATCC 33591D-5), Staphylococcus epidermidis (ATCC 12228D-5), Lactobacillus acidophilus (ATCC 4357D-5), Enterococcus faecalis (ATCC 700802D-5), Streptococcus pneumoniae (ATCC 6314D-5), Escherichia coli (ATCC 35638D-5), Klebsiella pneumoniae (ATCC BAA-1706D-5), Clostridium difficile (ATCCBAA-1382D-5), and Bifidobacterium infantis (ATCC 15697D-5).

Techniques: Marker

Journal: Journal of Neuroinflammation

Article Title: Probiotic treatment induces sex-dependent neuroprotection and gut microbiome shifts after traumatic brain injury

doi: 10.1186/s12974-025-03419-1

Figure Lengend Snippet: Schematic representation of the experimental design for acute and chronic TBI models with P Pan-Probiotic (PP) treatment. ( a ) Acute TBI Model: Mice received either water (VH) or a Pan-Probiotic (PP) cocktail containing Lactobacillus plantarum , L. reuteri , L. helveticus , L. fermentum , L. rhamnosus , L. gasseri , and L. casei via drinking water for 7 weeks prior to TBI induction. Acute assessments were performed 3 days post-injury (dpi), including microbiome sequencing, gut pathology analysis, histological examination, and blood collection. ( b ) Chronic TBI Model: Mice were pretreated with either water (VH) or the PP cocktail for 2 weeks before TBI or sham surgery. Post-TBI, treatment continued for 5 weeks, and outcomes were evaluated at multiple time points (7, 14, 21, and 35 dpi). Rotarod testing (R) was conducted to assess motor function, and behavioral tests, including the Elevated Plus Maze and Forced Swim Test, were performed to evaluate anxiety- and depressive-like behaviors. Additional analyses included microbiome sequencing, gut pathology, blood collection, histology, and RNAscope imaging at 35 dpi

Article Snippet: Lactobacillus helveticus (ATCC BAA-2840) , No , N/A , N/A.

Techniques: Sequencing, RNAscope, Imaging

Journal: Journal of Neuroinflammation

Article Title: Probiotic treatment induces sex-dependent neuroprotection and gut microbiome shifts after traumatic brain injury

doi: 10.1186/s12974-025-03419-1

Figure Lengend Snippet: The probiotic treatment promotes the selective persistence of Lactobacillus strains in the gut microbiome over time.

Article Snippet: Lactobacillus helveticus (ATCC BAA-2840) , No , N/A , N/A.

Techniques: